{"Absorption, Distribution and Excretion":"A rapid, specific and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the simultaneous determination of strychnine, brucine, strychnine N-oxide and brucine N-oxide in rat plasma. Plasma samples were pretreated via simple protein precipitation with methanol and ephedrine hydrochloride was used as internal standard. Chromatographic separation was carried out on an ZORBAX Eclipse XDB-C18 column (2.1 x 150 mm, 3.5 um) by gradient elution with methanol and 10mM ammonium acetate (adjusted to pH 4.0 with formic acid). The quantification of the analytes was performed by mass spectrometry with TurboIonSpray ionization (ESI) inlet in the positive ion multiple reaction monitoring (MRM) mode. The results showed that the calibration curve was linear in the concentration range of 0.510 to approximately 306.3 ng/mL for strychnine, brucine and 0.102 to approximately 306.0 ng/mL for strychnine N-oxide and brucine N-oxide, respectively. The intra- and inter-day precisions were less than 14.9%, and the accuracy ranged from 89.4 to 113% at three QC levels for the 4 analytes. The validated method was successfully applied to the pharmacokinetic study of strychnine, brucine, strychnine N-oxide and brucine N-oxide in rat plasma after oral administration of each monomer and the total alkaloids from Semen Strychni. After single oral administration of the total alkaloids from Semen Strychni at 4 dose levels, Cmax, AUC0-t of strychnine and brucine increased and were proportional to the oral doses. In comparative pharmacokinetics studies, no significant difference was found between each monomer and the total strychnos alkaloids on the pharmacokinetic parameters such as Cmax and AUC. Mean Cmax and AUC of strychnine and brucine were slight increased in the monomer groups in comparison to the total strychnos alkaloids groups, which suggested that some other alkaloids in the Semen Strychni may decrease the absorption of strychnine and brucine in body.","Adverse Effects":"Neurotoxin - Other CNS neurotoxin","Aliases":["Strychnine","Strychnidin-10-one","Sanaseed","Certox","Mole death","Strychninum","Gopher bait","Gopher-gitter","Boomer-rid","Hare-Rid","Mouse-tox","Kwik-kil","Ro-Dex","RCRA waste number P108","Stricnina","Strychnos","Mouse-nots","Mouse-rid","Dtxsid6023600","Chebi:28973","Dolco mouse cereal","NSC-5365","EPA Pesticide Chemical Code 076901","Pied piper mouse seed","Dtxcid503600","(4br,7as,8ar,13S,13ar,13bs)-5,6,7a,8,8a,11,13a,13b-octahydro-13H-13,14-ethano-7,9-methanooxepino(3,4-a)pyrrolo(2,3-d)carbazol-15-one","Strynchnos","NUX-VOMICA","(1R,11S,18S,20R,21R,22S)-12-oxa-8,17-diazaheptacyclo(15.5.2.0^(1,18).0^(2,7).0^(8,22).0^(11,21).0^(15,20))tetracosa-2,4,6,14-tetraen-9-one","(1R,11S,18S,20R,21R,22S)-12-oxa-8,17-diazaheptacyclo[15.5.2.0^{1,18}.0^{2,7}.0^{8,22}.0^{11,21}.0^{15,20}]tetracosa-2,4,6,14-tetraen-9-one","(4aR,5aS,8aR,13aS,15aS,15bR)-4a,5,5a,7,8,13a,15,15a,15b,16-decahydro-2H-4,6-methanoindolo(3,2,1-ij)oxepino(2,3,4-de)pyrrolo(2,3-h)quinolin-14-one","(4bR,7aS,8aR,13S,13aR,13bS)-5,6,7a,8,8a,11,13a,13b-octahydro-13H-13,14-ethano-7,9-methanooxepino[3,4-a]pyrrolo[2,3-d]carbazol-15-one","Nitrate, Strychnine","(4aR,5aS,8aR,13aS,15aS,15bR)-4a,5,5a,7,8,13a,15,15a,15b,16-decahydro-2H-4,6-methanoindolo(3,2,1-ij)oxepino(2,3,4-de)pyrrolo(2,3-h)quinoline-14-one","12-oxa-8,17-diazaheptacyclo(15.5.2.01,18.02,7.08,22.011,21.015,20)tetracosa-2,4,6,14-tetraen-9-one","200-319-7","(4aR,4a1R,5aS,8aR,8a1S,15aS)-4a1,5,5a,7,8,8a1,15,15a-octahydro-2H-4,6-methanoindolo[3,2,1-ij]oxepino[2,3,4-de]pyrrolo[2,3-h]quinolin-14(4aH)-one","Estricnina","Strychinos","NSC 5365","[3H]strychnine","(11S,18S,22S,1R,20R,21R)-12-oxa-8,17-diazaheptacyclo[15.5.2.0\u003c1,18\u003e.0\u003c2,7\u003e.0\u003c8 ,22\u003e.0\u003c11,21\u003e.0\u003c15,20\u003e]tetracosa-2,4,6,14-tetraen-9-one","Strychnin","Caswell No. 805","Unii-h9y79vd43j","Vauquline","Strychnine CRS","HSDB 2001","Ncgc00163241-01","SY9","Einecs 200-319-7","UN1692","RCRA waste no. P108","NCIStruc1_001823","NCIStruc2_000542","Schembl93798","GTPL347","orb105446","(19S)-Strychnidin-10-one","Chembl227934","GTPL2360","MEGxp0_001768","Schembl14029424","HMS2089C04","Tox21_112038","Bdbm50225707","Pdsp2_000441","SBB006463","Akos015955688","AC-8050","Cas-57-24-9","Ncgc00142530-02","Ncgc00186632-01","Ncgc00186632-02","Ncgc00489075-01","ST057252","NS00009135","S0249","Strychnine, PESTANAL(R), analytical standard","C06522","Ab01275436-01","Strychnine, European Pharmacopoeia (EP) Reference Standard"],"Biological Half-Life":"In nonfatal human poisoning cases, strychnine disappearance followed first-order kinetics with a half-life of 10 hr or 16 hr.","Boiling Point":"518 °","CAS":"57-24-9","Chemical Classes":"Biological Agents -\u003e Plant Toxins","ChemicalClasses":[],"Chirality":"absolute","Classes":["Toxin"],"Color/Form":"Brilliant, colorless cubes from chloroform-ether","Decomposition":"Hazardous decomposition products formed under fire conditions - Carbon oxides, nitrogen oxides (NOx).","Density":"1.36 at 68 °F (EPA, 1998) - Denser than water; will sink g/cm\u003csup\u003e3\u003c/sup\u003e","Ecotoxicity Values":"LD50; Species: /Anas platyrhynchos/ (Mallard duck) 6 month old males and females; oral 2.27 mg/kg (95% confidence limit: 1.26-4.11 mg/kg) /98% Strychnine alkaloid, NF/","Esters":[],"European Community (EC) Number":"200-319-7","Formating":[],"Health Effects":"Death may result from asphyxia or sheer exhaustion. (L1230)","HeavyAtomCount":25,"Human Drugs":"Pharmaceuticals","Human Toxicity Values":"Toxic strychnine blood concentration: 0.2 mg/dL; Lethal strychnine blood concentration: 0.9-1.2 mg/dL /From table/","IUPACName":"(4aR,5aS,8aR,13aS,15aS,15bR)-4a,5,5a,7,8,13a,15,15a,15b,16-decahydro-2H-4,6-methanoindolo[3,2,1-ij]oxepino[2,3,4-de]pyrrolo[2,3-h]quinolin-14-one","InChI":"InChI=1S/C21H22N2O2/c24-18-10-16-19-13-9-17-21(6-7-22(17)11-12(13)5-8-25-16)14-3-1-2-4-15(14)23(18)20(19)21/h1-5,13,16-17,19-20H,6-11H2/t13-,16-,17-,19-,20-,21+/m0/s1","InChIKey":"QMGVPVSNSZLJIA-FVWCLLPLSA-N","Interactions":"Theanine, an additive, holds several effects on the central nervous system without toxicity and affects CNS drugs. Theanine bilaterally alters beta wave of the EEG with or without caffeine and pentobarbital-induced locomotor activity. Theanine also enhances hypnosis of pentobarbital sodium (PB) and antidepression of midazolam, suggesting there are complicated interactions between theanine and CNS drugs. On the other side, theanine induces glycine release. Glycine potentiates the strychnine toxicity via NMDA receptor activation. Moreover, PB facilitates GABAA receptor activation by GABA, and it is commonly prescribed for strychnine poison. However, what the role that theanine plays in the anticonvulsion of PB against strychnine poison is still unknown. Theanine, pentobarbital sodium or strychnine was injected intraperitoneally. EEG was monitored by BIOPAC 16 EEG amplifiers. LD50 of strychnine and hypnotic ED50 of pentobarbital sodium with or without theanine for mice were tested according to Bliss' case.  (1) Theanine enhanced the strychnine toxicity. Both theanine and strychnine 1.0?mg/kg increased the power of the beta wave. Theanine aggravated that of strychnine 1.0?mg/kg. Theanine attenuated the LD50 of strychnine. (2) Theanine enhanced the anticonvulsion of PB. Theanine increased the power of alpha, beta wave and decreased hypnotic ED50 of PB; PB attenuated strychnine-induced EEG excitation and mortality with or without theanine, and theanine enhanced the effects of PB. Further, theanine enhanced the anticonvulsion of PB dose-dependently against the strychnine toxicity but not the lethal toxicity of strychnine. These results indicated theanine interacted with PB and strychnine. Theanine enhanced both the strychnine toxicity and anticonvulsion of PB against strychnine poison.","KEGG Entries":[{"Id":"D10470","Interactions":[],"Synonyms":["Strychnine nitrate"]},{"Id":"D10470","Interactions":[],"Synonyms":["Strychnine nitrate"]}],"MeSH Headers":[{"Id":"M0020628","Link":"https://id.nlm.nih.gov/mesh/M0020628.html","Name":"Strychnine","Ref":137},{"Id":"DescTree","Link":"https://www.nlm.nih.gov/mesh/meshhome.html","Name":"MeSH Tree","Ref":139},{"Id":"PubMed from MeSH","Link":"https://www.nlm.nih.gov/mesh/meshhome.html","Name":null,"Ref":165},{"Id":"M0005136","Link":"https://id.nlm.nih.gov/mesh/M0005136.html","Name":"Convulsants","Ref":166},{"Id":"M0017100","Link":"https://id.nlm.nih.gov/mesh/M0017100.html","Name":"Poisons","Ref":167},{"Id":"M0027996","Link":"https://id.nlm.nih.gov/mesh/M0027996.html","Name":"Glycine Agents","Ref":168}],"MeSH Pharmacological Classification":[{"Id":"M0005136","Link":"https://id.nlm.nih.gov/mesh/M0005136.html","Name":"Convulsant","Ref":166},{"Id":"M0017100","Link":"https://id.nlm.nih.gov/mesh/M0017100.html","Name":"Poison","Ref":167},{"Id":"M0027996","Link":"https://id.nlm.nih.gov/mesh/M0027996.html","Name":"Glycine Agent","Ref":168}],"Mechanism of Action":"Strychnine is a strong convulsant. The alkaloid excites the CNS by specifically antagonizing the inhibitory neurotransmitter amino acid, glycine, at postsynaptic receptors. Inhibitory glycine receptors are abundant in the spinal cord and brain stem where they are mainly involved in regulation of motor functions. Strychnine-binding glycine receptors were also found in the cortex, as well as in the auditory and visual systems. When inhibition is blocked, ongoing neuronal excitability is increased and sensory stimuli produce exaggerated reflex effects. Glycine receptors in higher brain centers such as the substantia nigra, neostriatum, and hippocampus are commonly insensitive to strychnine, explaining why strychnine symptoms are largely spinal in origin. Strychnine can also depress nicotinic-cholinergic responses through interaction with nicotinic receptors and at high concentrations in vitro binds to other receptors as well.","Melting Point":"514 to 554 °F (EPA, 1998)","Metabolism/Metabolites":"Within a few minutes of ingestion, strychnine appears unchanged in the urine, but the major route for removal and detoxification is oxidative hepatic metabolism.","MolecularFormula":"C\u003csub\u003e21\u003c/sub\u003eH\u003csub\u003e22\u003c/sub\u003eN\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e","MolecularWeight":"334.4 g/mol","Non-Human Toxicity Values":"LD50 Rat iv 0.96 mg/kg (slow infusion)","Odor":"Odorless","Opticalactivity":"UNSPECIFIED","Physical Description":"Strychnine appears as colorless, transparent crystals or white crystalline powder. Has no odor. Used for destroying rodents and predatory animals and for trapping fur-bearing animals. (EPA, 1998)","PubChemId":441071,"Records":{"UNII":{"Impurities":[]}},"RefChem":"6264","RefCount":3,"RefCur":"","References":[{"Name":"Wikipedia","Urls":[{"Link":"https://en.wikipedia.org/wiki/Strychnine","Name":"Strychnine","Sub":false}]},{"Name":"Wikidata","Urls":[{"Link":"https://www.wikidata.org/wiki/Q194406","Name":"Strychnine","Sub":false}]},{"Name":"DrugBank","Urls":[{"Link":"https://go.drugbank.com/DB15954","Name":"Strychnine","Sub":false}]},{"Name":"PubChem","Urls":[{"Link":"https://pubchem.ncbi.nlm.nih.gov/compound/441071","Name":"Strychnine","Sub":false}]},{"Name":"Common Chemistry","Urls":[{"Link":"https://commonchemistry.cas.org/detail?cas_rn=57-24-9","Name":"Strychnine","Sub":false}]},{"Name":"KEGG","Urls":[{"Link":"https://www.kegg.jp/entry/C06522","Name":"Strychnine","Sub":false}]},{"Name":"UNII","Urls":[{"Link":"https://gsrs.ncats.nih.gov/ginas/app/ui/substances/H9Y79VD43J","Name":"Strychnine","Sub":false}]},{"Name":"EPA DSSTox","Urls":[{"Link":"https://comptox.epa.gov/dashboard/chemical/details/DTXSID6023600","Name":"Strychnine","Sub":false}]}],"Refs":["National Center for Biotechnology Information. PubChem Compound Summary for CID 441071, Strychnine. Accessed April 15, 2026. \u003ca href=https://pubchem.ncbi.nlm.nih.gov/compound/441071\u003ehttps://pubchem.ncbi.nlm.nih.gov/compound/441071\u003c/a\u003e","U.S. Food and Drug Administration; National Center for Advancing Translational Sciences. Strychnine. UNII: H9Y79VD43J. Global Substance Registration System. Accessed April 15, 2026. \u003ca href=https://gsrs.ncats.nih.gov/ginas/app/beta/substances/H9Y79VD43J\u003ehttps://gsrs.ncats.nih.gov/ginas/app/beta/substances/H9Y79VD43J\u003c/a\u003e"],"Reported Fatal Dose":"The typical lethal dose of strychnine is between 50 and 100 mg for adults and 15 and 30 mg for children; however, much higher doses were reportedly tolerated.","SMILES":"C1CN2CC3=CCO[C@H]4CC(=O)N5[C@H]6[C@H]4[C@H]3C[C@H]2[C@@]61C7=CC=CC=C75","SaltData":[{"AcidCount":1,"Amine":"Strychnine","AmineCount":2,"Formula":"OS(=O)(=O)O","Name":"hemisulfate","RName":"sulfate","Structure":"\u003csvg xmlns=\"http://www.w3.org/2000/svg\" preserveAspectRatio=\"none\" style=\"display:block\" viewBox=\"0 0 156.243 87.729\"\u003e\u003crect width=\"100%\" height=\"100%\" fill=\"#fff\"/\u003e\u003cdesc\u003eGenerated by the Chemistry Development Kit (http://github.com/cdk)\u003c/desc\u003e\u003cg fill=\"#ff0d0d\" stroke=\"#000\" stroke-linecap=\"round\" stroke-linejoin=\"round\" 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0-.929-.387-1.453-.381-.524-1.208-.524-.833 0-1.226.524t-.393 1.453\" class=\"atom\"/\u003e\u003cpath fill=\"#3050f8\" stroke=\"none\" d=\"M43.723 55.48h-.721l-2.619-4.066h-.03l.03.596q.024.357.024.732v2.738h-.566v-4.899h.715l2.607 4.054h.03l-.018-.327-.024-.477q-.006-.262-.006-.482v-2.768h.578z\" class=\"atom\"/\u003e\u003cpath stroke=\"#3050f8\" d=\"m37.763 4.225-5.395 2.017M44.356 3.039l13.543.12\" class=\"hi\"/\u003e\u003cpath stroke=\"#ff0d0d\" d=\"m89.951 57.679 4.599-3.341\" class=\"hi\"/\u003e\u003cpath stroke=\"#3050f8\" d=\"m43.527 56.748 2.372 5.054M38.518 51.9l-5.547-1.92\" class=\"hi\"/\u003e\u003c/g\u003e\u003c/g\u003e\u003c/svg\u003e","Subjective Effects":null,"Taste":"Very bitter metallic taste","Therapeutic Uses":"/EXPL THER/ Strychnine and brucine from the seeds of the plant Strychnos nux vomica have been shown to have interesting pharmacological effects on several neurotransmitter receptors. In this study, we have characterized the pharmacological properties of strychnine and its analogs on human Na(v)1.5 channels to assess their potential therapeutic advantage in certain arrhythmias. Among the eight alkaloids, only strychnine and icajine exhibited inhibition potency on the Na(v)1.5 channel with the half-maximum inhibition (IC(50)) values of 83.1 uM and 104.6 uM, respectively. Structure-function analysis indicated that the increased bulky methoxy groups on the phenyl ring or the negatively charged oxygen atom may account for this lack of inhibition on the Na(v)1.5 channel. Strychnine and icajine may bind to the channel by cation-pi interactions. The substitution with a large side chain on the phenyl ring or the increased molecular volume may alter the optimized position for the compound close to the binding sites of the channel. Strychnine and icajine bind to the Na(v)1.5 channel with a new mechanism that is different from TTX and local anesthetics. They bind to the outer vestibule of the channel pore with fast association and dissociation rates at resting state. Strychnine and icajine had little effect on steady-state fast inactivation but markedly shifted the slow inactivation of Na(v)1.5 currents toward more hyperpolarized potentials. The property of icajine influencing slow-inactivated state of Na(v)1.5 channel would be potential therapeutic advantages in certain arrhythmias.","Title":"Strychnine","Toxicity Data":"LD50: 2350 ug/kg (Oral, Rat) (T14)\nLD50: 1100 ug/kg (Intraperitoneal, Rat) (T14)\nLD50: 1200 ug/kg (Subcutaneous, Rat) (T14)\nLD50: 582 ug/kg (Intravenous, Rat) (T14)","Treatment":"Treatment of strychnine poisoning involves an oral application of an activated charcoal infusion to absorb any poison within the digestive tract that has not yet been absorbed into the blood. Anticonvulsants such as phenobarbital or diazepam are administered to control convulsions, along with muscle relaxants such as dantrolene to combat muscle rigidity. (L1228)","UNII":"H9Y79VD43J","Wikidata":"Q194406","Wikipedia":"Strychnine","XLogP":1.9,"pH":"pH 9.5, saturated solution"}